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Fast, Accurate and Economic Solution for Scientific Discoveries
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Ellman's Reagents
Product Name: 2-PDS
Product Code: P016-10 Unit: 1 g
Description:
Chemical Name: 2,2-Dithiodipyridine
Appearance: white or pale yellow powder
m.p.: 56-60 ºC
Molar
absorptivity: >14,000 (235 nm)
Sulfated ash: <0.1 %
Solubility:
100 mg/ml methanol
Oxidation Reaction
2-PDS and 4-PDS are
used for the photometric determination of thiol groups in biological samples.
These compounds react with the thiol groups at pH 5 to form mercaptopyridine.
The maximum wavelengths of 2-Mercaptopyridine and 4-Mercaptopyridine are 343 nm
and 324 nm, respectively.
Product Name: 4-PDS
Product Code: P017-10 Unit: 1 g
Description:
Chemical Name: 4,4'-Dithiodipyridine
Appearance: white or pale yellow crystalline powder
m.p.: 73-78
ºC
Molar absorptivity: >15,000 (246 nm)
Sulfated ash: <0.1 %/
Solubility: 100 mg/ml methanol
Oxidation Reaction
2-PDS and
4-PDS are used for the photometric determination of thiol groups in biological
samples. These compounds react with the thiol groups at pH 5 to form
mercaptopyridine. The maximum wavelengths of 2-Mercaptopyridine and
4-Mercaptopyridine are 343 nm and 324 nm, respectively.
Product Name: DTNB
Product Code: D029-10 Unit: 1 g
Description:
Chemical Name: 5,5-Dithiobis(2-nitrobenzoic acid)
Appearance: pale yellow crystalline powder
m.p.: 237-247 ºC
(dec.)
Molar absorptivity: >12,000(305 nm)
Absorbance: <0.195 (420
nm)
Sulfated ash: <0.1 %
Loss on drying: <1%
Solubility: 10
mg/ml ethanol, 2 mg/ml 50 mM phosphate buffer pH 7.2
Oxidation
Reaction
DTNB is known as Ellman? reagent. It is used for the colorimetric
determination of thiol groups in biological samples. It is fairly soluble in
water. Colorless DTNB is converted to yellow 5-Mercapto-2-nitrobenzoic acid in
the presence of thiol compounds. As 5-Mercapto-2-nitrobenzoic acid has an
absorption maximum at 412 nm, the absorption spectrum of DTNB does not interfere
with thiol detection.
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