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Detergent Screening Set

Alkylmaltosides,

Alkylthioglucoside

Alkylthiomaltoside

Alkylglucoside

Sucrose alkylate

Cholamide, Nonionic detergent

Cholamide, Zwitter ion

Cholate

Gluconamides

Sucrose Cholate

Protein Quantification Kit – Rapid  ( Bradford Method )

 

Detergent Screening Set

Code

DS04-10 (first choice)

DS05-10 (for crystallization)

Contents

CHAPS (200 mg x 1)
n-Dodecyl-beta-D-maltoside (200 mg x 1)
n-Octyl-beta-D-glucoside (200 mg x 1)
Sodium cholate (purified) (200 mg x 1)
Sucrose monolaurate (200 mg x 1)

n-Decyl-beta-D-maltoside (200 mg x 1)
n-Dodecyl-beta-D-maltoside (200 mg x 1)
n-Octyl-beta-D-glucoside (200 mg x 1)
n-Octyl-beta-D-maltoside (200 mg x 1)
MEGA-10 (200 mg x 1)

Detergent Screening Sets are used to find the best detergent for an application such as enzyme stabilization, specific membrane protein isolation, assay development, protein crystallization, protein refolding and so on.

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Detergents

Alkylmaltosides,

Product Name: n-Decyl-beta-D-maltoside ( n-Decyl-beta-maltopyranoside )

Unit

Product Code

1 g

D382-10

5 g

D382-12

 

Product Name: n-Dodecyl-beta-D-maltoside ( n-Dodecyl-b-D-maltopyranoside )

Unit

Product Code

1 g

D316-10

5 g

D316-12

n-Dodecyl-b-D-maltoside is a non-ionic detergent. It has a glyco-chain in its lipophilic site that is similar to the one in n-Octyl-b-D-glucoside. Using this detergent, Dr. Van Aken and others succeeded in solubilizing the active cytochrome oxidase from mitochondria. Its CMC value is 0.17 mM.

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Alkylthioglucoside

Product Name: n-Heptyl-beta-D-thioglucoside ( n-Heptyl-b-D-thioglucopyranoside )

Unit

Product Code

1 g

H015-10

5 g

H015-12

n-Heptyl-b-D-thioglucoside is an effective non-ionic detergent for the solubilization of membrane proteins such as n-Octyl-b-D-glucoside. Though n-Octyl-b-D-glucoside is degraded by b-glycosidase, thioether is not. This means that n-Heptyl-b-D-thioglucoside is effective for the solubilization of samples with b-glycosidase activity. The CMC value of n-Heptyl-b-D-thioglucoside is 30 mM. It is soluble in aqueous solutions at 4 ºC making it suitable for the solubilization of membrane proteins at low temperatures.

Product Name: n-Octyl-beta-D-thioglucoside ( n-Octyl-b-D-thioglucopyranoside )

Unit

Product Code

 

1 g

O003-10

5 g

O003-12

n-Octyl-b-D-thioglucoside is similar to n-Octyl-b-D-glucoside. After solubilization, it can be separated from phospholipid or protein by dialysis. It does not interfere with enzyme activities. It is more stable than n-Octyl-b-D-glucoside, and is not affected by esterase. Its CMC value is 9 mM

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Alkylthiomaltoside

Product Name: n-Nonyl-beta-D-thiomaltoside ( n-Nonyl-b-D-thiomaltopyranoside )

Unit

Product Code

1 g

N373-10

n-Nonyl-b-D-thiomaltoside is a newly developed detergent whose lipophilic moiety is maltose. This reagent is suitable for isolating proteins inside of cell membranes. The activity of protein isolated using n-Nonyl-b-D-thiomaltoside is higher than those isolated using n-Octyl-b-D-thioglucoside or n-Heptyl-b-D-thioglucoside. CMC value is 2.4 mM

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Alkylglucoside

Product Name: n-Octyl-beta-D-glucoside ( n-Octyl-b-D-glucopyranoside )

Unit

Product Code

1 g

O001-10

5 g

O001-12

n-Octyl-b-D-glucoside has definite advantages over the conventional non-ionic detergents as a membrane protein solubilizer. It can be easily separated by dialysis, and has very little interaction with membrane proteins. Its CMC value is 25 mM. It is strongly recommended for studies of ATPase or NADH dehydrogenase.

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Sucrose alkylate

  Product Name:Sucrose monocaprate (b-D-Fructopyranosyl-a- D-glucopyranoside monodecanoate )

Unit

Product Code

1 g

S021-10

5 g

S021-12

 

  Product Name: Sucrose monolaurate(b-D-Fructopyranosyl-a-D-glucopyranoside monododecanoate)

Unit

Product Code

1 g

S023-10

5 g

S023-12

Sucrose monocaprate and Sucrose monolaurate are new non-ionic detergents that contain sucrose as the hydrophilic group, and caprate or laurate as the hydrophobic group. These detergents are designed to solubilize membrane proteins (e.g., enzymes, receptors and transport carriers) without causing denaturation. These detergents can be removed by dialysis because of their high hydrophilicity and CMC values. Due to their low absorbance at 280 nm, they can be used for the quantification of proteins by measuring absorbance.

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Cholamide, Nonionic detergent

Product Name: BIGCHAP ( N,N-Bis(3-D-gluconamidopropyl)cholamide )

Unit

Product Code

1 g

B043-10

5 g

B043-12

BIGCHAP and deoxy-BIGCHAP are non-ionic detergents based on a cholic acid and a bisglucon-amidopropyl group. The CMC values are 2.9 mM and 1.4 mM, respectively. BIGCHAPS and deoxyBIGCHAP are easily removed by dialysis, and their absorption in the UV region is very low.

Product Name: deoxy-BIGCHAP ( N,N-Bis(3-D-gluconamidopropyl)deoxycholamide )

Unit

Product Code

1 g

D045-10

5 g

D045-12

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Cholamide, Zwitter ion

Product Name: CHAPS ( 3[(3-Cholamidopropyl)dimethylammonio]propanesulfonic acid )

Unit

Product Code

1 g

C008-10

5 g

C008-12

25 g

C008-14

 

Product Name: CHAPSO(3-(3-Cholamidopropyl)dimethylammonio-2-hydroxypropanesulfonic acid)

Unit

Product Code

1 g

C020-10

5 g

C020-12

CHAPS and CHAPSO are zwitter ionic detergents, and have cholic acid and sulfobetaine moieties in their structures. Their low background absorbance in the UV region is an attractive feature for the UV monitoring of membrane proteins. The CMC values of both CHAPS and CHAPSO are 8 mM.

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Cholate

Product Name: Sodium cholate (purified) ( Cholic acid, sodium salt, monohydrate )

Unit

Product Code

5 g

C321-10

25 g

C321-12

Sodium cholate is an anionic detergent with 14 mM CMC. Since three hydroxy groups are located on a steroid ring and one carboxylic group is at the terminus of the molecular structure, there are no definitive lipophilic or hydrophilic regions in the molecule. In general, cholate detergents can be easily removed by dialysis because of their fairly small micelle sizes.

Product Name: Sodium deoxycholate (for protein crystallization) ( Deoxycholic acid, sodium salt, monohydrate )

Unit

Product Code

1 g

D520-10

5 g

D520-12

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Gluconamides

Product Name: MEGA-10 ( n-Decanoyl-N-methylglucamide )

Unit

Product Code

1 g

M016-10

5 g

M016-12

Glucamide detergents, MEGA8/9/10 are non-ionic, transparent in the UV region, and possess relatively high CMC values. Therefore, they are ideal for use as membrane protein solubilizers. The CMC values for these detergents are between 7 mM and 25 mM.

Product Name: MEGA-8 ( n-Octanoyl-N-methylglucamide )

Unit

Product Code

1 g

M014-10

5 g

M014-12

 

Product Name: MEGA-9 ( n-Nonanoyl-N-methylglucamide )

Unit

Product Code

1 g

M015-10

5 g

M015-12

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Sucrose Cholate

Product Name: Sucrose Monocholate (mixture)

Unit

Product Code

1 g

S260-10

5 g

S260-12

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Related Product

Protein Quantification Kit – Rapid  ( Bradford Method )

Product Code  PQ01-12  2500 Tests 

Advantages:

  • Complete, Ready-to-Use, Microplate Assay Kit

  • High Sensitivity (5µg/ml to 2000µg/ml, BSA)

  • Short Incubation Time (30 seconds)

  • No Use of Heavy Metal Ions

2500 tests
CBB Solution: 1000 ml x 1 bottle
BSA Standard(4000 µg/ml): 1.5 ml x 2 vials

Mechanism of Protien Detection

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