New Alternatives to Ethidium Bromide

SYBR Green I ( for electrophoresis )

SYBR Green I is DNA fluorescence dye with high sensitivity, suitable for various gel analysis. Simple operation: no need to decolor or scoure. At least 20pg DNA may be detected, 25—100 times more sensitive than EB stain method. Fluorescent signal may be enhanced up to 800—1000 times when SYBR Green I is combined with dsDNA. Fluorescent signal from gel samples are so strong that weak signals from background could be eliminated. SYBR Green may be suitable for various gel analysis.

SYBR Green I is fit for different kinds of gel electrophoresis methods: agarose gel, PAGE gel, pulsed field gel electrophoresis, and capillary electrophoresis, ect.

SYBR Green I has high affinity with dsDNA, so post-electrophoresis dyeing is feasible, with no inhibitory effect on traditional enzymes (such as: Taq, reverse transtcriptase, interior contactase, T4 DNA ligases, ect). Besides, compared with EB, mutagenicity of SYBR Green I reduced enormously.

GeneFinder

GeneFinder is a new type of nucleic acid dye developed by us. With virtues of high sensitivity and safety, it’s a excellent substitution for EB as nucleic acid electrophoresis dye.

Different to high toxic EB, GeneFinder is cyanine dye with low toxicity and high safety, to protect lab-operators and environment effectively.

Fluorescent signals may be enhanced up to 800-1000 times when GeneFinder is combined with dsDNA and sensitivity is about 10 times higher than EB dye. Nearly the same as SYBR Green I of Molecular Probe, Co, US.

The maximum absorption peak of GengFinder dye is 470nm. Nucleic acid combined with dye emit green fluorescent light, which may be observed by BlueShield visible light gel analyzer. UV gel analyzer may also be used, but with poor effect.

GoldView

GoldView^™ is a new, safe nucleic acid stain instead of tranditional ethidium bromide (EB) for detecting double- stranded DNA, single-stranded DNA, and RNA in agarose gel. It emits green fluorescence when bound to dsDNA and red  fluorescence when bound to ssDNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at approximately 290 nm and one at approximately 490nm. GoldView^™ is as sensitive as EB, and its protocol is the same way to EB. 

Compared to EB known as strong mutagen, GoldView^™ cause much fewer mutations is the Ames test. In addition, GoldView^™has a negative test in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. So it is wise to choose GoldView^™ instead of EB for detecting nucleic acid in  agarose gels.