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The enzyme 5-lipoxygenase peroxidizes arachidonic acid to form 5-HPETE, which is converted to its epoxide derivative leukotriene A4 (LTA4). LTA4 may be subsequently hydrolyzed to LTB4 or may be converted to LTC4. 5-Lipoxygenase products influence a variety of biological processes. LTB4 is a potent chemotactic agent for polymorphonuclear leukocytes, whereas LTC4 and its hydrolysis product, LTD4, are potent bronchoconstrictors and mediators of allergic hypersensitivity. These products are also increased in hypersensitivity diseases such as asthma and thus may have significant roles in the pathology of such diseases. 5-Lipoxygenase products may also be involved in cell regulation and maturation as substantial amounts of 5-lipoxygenase are produced when HL-60 leukemic cells are induced to differentiate. 5-Lipoxygenase represents a key site for the regulation of numerous biological events, thus the development of inhibitors and the identification of potential activators of this enzyme are very active areas of research.
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cDNA Probe for 5-Lipoxygenase This large fragment of the cDNA encoding human 5-lipoxygenase specifically hybriizes to mammalian 5-lipoxygenase, but not with 12-lipoxygenases or 5-lipoxygenases Product No.R 79 |
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EZ-Prime Human 5-LOX Primers This oligonuceotide primer set is designed for specific PCR amplification of human 5-LOX message, giving an amplimer of 510 bp. One vial is sufficient for > 100 amplification reactions, and is provided with complete instructions and optimal experimental conditions. Product No.EZ 05 |
The existence of an enzyme that produces 8-HETE in mouse
skin was described by Furstenberger and coworkers in 1986 (1). Brash and
coworkers (2) have recently reported the cloning of this enzyme, which is
strongly induced by topical application of phorbol esters. The cDNA for 8-lox
encodes a 76 kDa protein with approximately 40% homology to most lipoxygenases.
However it exhibits 76% homology to a recently cloned 15-lipoxygenase isoform
expressed in some human tissues (3). The physiologic and pathophysiologic
roles of this enzyme, which are also detectable in brain, remain to be
determined.
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cDNA Probe for 8-Lipoxygenase This large fragment of the cDNA encoding murine 8-lipoxygenase specifically hybridizes to mRNA encoding this enzyme, but not with mRNA encoding 5-lipoxygenase, 12-lipoxygenase or 15 lipoxygenase. Product No. R 08 |
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Anti-8-Lipoxygenase (Polyclonal) This rabbit antibody is specific for 8-lipoxygenase in mice. In human tissues, it is specific for 15-lipoxygenase form-2. This antibody is useful for enhanced chemiluminescence, immunohistochemisty, immunoprecipitation and ELISA applications. Product No. LX 08 |
12-Lipoxygenase
Three forms of 12-lipoxygenase have been identified, "leukocyte-type" and "platelet-type" (12(S) LOXs), and a 12(R) LOX in skin. These differ in tissue localization, substrate specificities, immunogenicities and sequence homology. The leukocyte-type 12-lipoxygenases have also been found in other tissues, including the rat pineal gland and human adrenal gland. The platelet-type 12-lipoxygenases have thus far been found only in platelets and human epidermal cells. Because of the significant regulatory effects of 12(S)-HPETE and 12(S)-HETE, therapeutic agents which either activate or inhibit 12-lipoxygenase may be useful in treatment of artherosclerosis, hypertensive disorders, diabetes, and metastatic diseases. Both isoforms of 12-lipoxygenase have recently been cloned. (Chen, et al., J. Biol. Chem. 269:13979-13987, 1994.)
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12-Lipoxygenase (Platelet-Type) This partially purified preparation of native human platelet 12-lipoxygenase has negligible enzymatic activity, but is useful as a standard for Western blotting. Each vial contains sufficient enzyme for at least 10 Western blots. Product No. LX 12PS |
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12-Lipoxygenase (Leukocyte-Type) This stabilized and partially purified porcine leukocyte enzyme has a specific activity of approximately 50 units/mg protein. The enzyme is suspended in 20 mM Tris pH 7.2 containing 0.5 mM EDTA, PMSF, DTT, and40% glycerol. Stable at -70°C. Labile to freezing and thawing. One unit is the amount of enzyme that catalyzes the conversion of one nanomole of arachidonic acid to 12-HPETE during a minute assay at roomtemperature. (Biochem. Biophys. Acta. 713:638-646, 1982.) Product No.LX 12L |
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cDNA Probe for Murine Platelet-Type 12-Lipoxygenase This large fragment of the cDNA encoding murine platelet-type 12-lipoxygenase is useful for the preparation, by random priming or nick translation, of labeled probes that can subsequently be used to screen tissues for differential expression of this 12-LOX isoform. Product No.MP 12 |
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cDNA Probe for Human Platelet-Type 12-Lipoxygenase This large fragment of the cDNA encoding human platelet-type 12-lipoxygenase cDNA probe should cross-hybridize with mammalian platelet-type 12-lipoxygenases, but not with other lipoxygenases, including leukocyte-type lipoxygenases. Product No.R 84 |
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cDNA Probes for Murine Leukocyte-Type 12-Lipoxygenase This large fragment of the cDNA encoding murine leukocyte-type 12-lipoxygenase is useful for the preparation, by random priming or nick translation, of labeled probes that can subsequently be used to screen tissues for differential expression of this recently reported 12-LOX isoform. (J. Biol. Chem. 269:13979-13987, 1994.) Product No.ML 12 |
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cDNA Probe for 12(R) Lipoxygenase 12(R) LOX isoform found in skin.This large 2.5 kb message encoding 12 (R) Lipoxygenase is useful for the preparation, by random priming or nick translation, of labeled probes that can subsequently be used to screen tissues for differential expression of this 12-LOX isoform. (Proc. Natl. Acad. Sci. 95/12:6744-6749, 1998.) Product No.R-16 |
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EZ-Prime Human Platelet 12-LOX Primers This oligonuceotide primer set is designed for specific PCR amplification of human platelet 12-LOX message, giving an amplimer of 359 bp. One vial is sufficient for > 100 amplification reactions, and is provided with complete instructions and optimal experimental conditions. (J. Biol. Chem. 268:16443-16448, 1993.) Product No.EZ 13 |
15-Lipoxygenase
The enzyme 15-lipoxygenase produces 15-HETE, which can be metabolized to the trihydroxy compounds lipoxin A4 (LXA4) and lipoxin B4 (LXB4), as well as 5(S),15(S)-DiHETE. The lipoxins exhibit a range of biological activities. LXA4 has been reported to increase kidney glomerular filtration rate and to provoke contraction of guinea pig lung. Two 15-lipoxygenase enzymes have been cloned and characterized from human tissues and are differentially expressed. One isoform (15-LOX form-1) is expressed in a variety of cell types including neutrophils, eosinophils and macrophages. Expression of the second isoform (15-LOX form-2), which was identified and cloned very recently (Brash, Boeglin, et al., Proc. Natl. Acad. Sci (USA) 94:6148-6152, 1997.), appears restricted to lung, hair roots, prostate, and cornea.
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cDNA Probe for 15-Lipoxygenase Form-1 This fragment of the cDNA encoding human 15-LOX form-1 specifically cross-hybridizes with the 15-lipoxygenase isoform from other mammals. At low stringency, it may hybridize with some leukocyte-type lipoxygenases. Product No. R
89 |
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EZ-Prime 15-LOX Primers This oligonuceotide primer set is designed for specific PCR amplification of 15-LOX form-1 message, giving an amplimer of 952 bp. One vial is sufficient for > 100 amplification reactions, and comes with complete instructions and optimal experimental conditions. Product No. EZ 15 |
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Anti-15-Lipoxygenase Form-2, Polyclonal This rabbit antibody reacts specifically with 15-lipoxygenase form-2 in human tissues and 8-lipoxygenase in murine tissues. This antibody is useful for enhanced chemiluminescence, immunohistochemisty, immunoprecipitation and ELISA applications. Product No. LX 25 |
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cDNA Probe for Human 15-Lipoxygenase Form-2 This large fragment of the cDNA encoding human 15-lipoxygenase form-2 specifically hybridizes to mRNA encoding this enzyme, but not with 5-lipoxygenase, 12-lipoxygenases or 15 lipoxygenase form-1 mRNA. Product No. R 15 |
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EZ-Prime 15-LOX Form-2 Primers This oligonuceotide primer set is designed for specific PCR amplification of human 15-LOX form-2 message. One vial is sufficient for 100 amplification reactions and comes with complete instructions for optimal experimental conditions. Product No.EZ 16 |
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Product No. |
Description |
Inhibition |
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BL 10 |
AA-861 |
potent inhibitor of 5-lipoxygenase |
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BL 15 |
Baicalein |
12-lipoxygenase |
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BL 20 |
Caffeic Acid (3-4-Dihydroxycinnamic Acid) |
5-lipoxygenase |
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BL 25 |
CDC (Cinnamyl-3, 4-Dihydroxy-a-Cyanocinnamate) |
C19H15NO4 is a general lipoxygenase inhibitor is more potent for 12-lipoxygenase (IC50 = 63 nM) than 5-lipoxygenase (IC50 = 1.89 µM) or 15-lipoxygenase (IC50 = 3.33 µM) |
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BL 30 |
Gossypol |
2-lipoxygenase (IC50 = 0.7 µM)/ 15-lipoxygenase (IC50 = 0.3 µM) |
Leukotriene A4 Hydrolase
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cDNA Probe for Leukotriene A4 Hydrolase LTA4 hydrolase is a bifunctional metalloenzyme that hydrolyzes LTA4 to the pro-inflammatory substance LTB4 and also has peptidase activity. This large fragment of the cDNA encoding human LTA4 hydrolase specifically hybridizes with LTA4 hydrolase from most mammals Product No.LT 10 |
Oxford Biomedical Research provides highly reliable competitive ELISA kits for the quantification of major lipoxygenase metabolites. For additional information about obtaining quantitative data in solid phase competitive ELISA assays, please see the review article by Sasaki and Mitchell.
In general, eicosanoids must first be extracted from serum, using either liquid or solid phase extraction techniques, prior to immunoassay. In contrast, levels of eicosanoids in urine and tissue culture supernatant may often be assayed directly following dilution with buffer. Recommended extraction procedures are included in the instructions provided with each kit. The following extraction procedure is recommended, for example, prior to the analysis of prostaglandin E2 in complex biological samples.
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Leukotriene B4 EIA Kit LTB4 is the most potent chemotactic substance ever described for leukocytes. Using this competitive ELISA, LTB4 in urine and tissue culture supernatants can be assayed directly, whereas LTB4 should be extracted from plasma prior to analysis. Product No.EA 35 Instruction Booklet |
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Leukotriene C4 EIA Kit Leukotriene C4 (LTC4) is a potent mediator of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability, and epithelial mucous secretion. This competitive ELISA assay is based on the competition between the LTC4 present in your sample and the LTC4-enzyme conjugate for a limited number of antibody binding sites. Product No. EA 38 Instruction Booklet |
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Leukotriene C4/D4/E4 EIA Kit Leukotriene C4/D4/E4 are potent mediators of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability, and epithelial mucous secretion. They are derived from arachidonic acid through the 5-lipoxygenase pathway. The monoclonal antibody in this kit recognizes LTC4, LTD4 and LTE4 and allows for the quantitation of all of these potent leukotrienes in a single assay. This ELISA provides quantitative analysis of LTC4/D4/E4 levels in biological fluids. This is a competitive ELISA assay. It is based on the competition between the LTC4/D4/E4 present in your sample and the enzyme conjugate for a limited number of antibody binding sites. Product No.EA 39 Instruction Booklet |
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15-Epi-Lipoxin A4 EIA Kit Lipoxins are among a relatively new class of eicosanoids that are
derived from arachidonate and known to be prevalent in airway tissues of
asthmatics and other lung disease patients. This demonstrates that lipoxins are
naturally occurring molecules and closely associated with eosinophil-related
pathways. While their exact roles are not fully understood, both Lipoxin A4
(LXA4) and15-Epi-Lipoxin A4 (15-Epi-LXA4) have proven to be involved in
anti-inflammatory responses. The LXA4, is however, not effective in abolishing
allergen induced eotaxin while the analog 15-Epi-Lipoxin A4 has been shown to be
effective. Furthermore, the 15-Epi-LXA4 has been established as an inhibitor of
eosinophil localization in allergen sensitized tissue making it a molecule of
great interest for tissue inflammation studies. Product No.EA 46. |
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Lipoxin A4 EIA Kit Lipoxin A4 (LXA4) is a biologically active product of the lipoxygenase pathway. LXA4 stimulates leukocyte chemotaxis without aggregation and inhibits natural killer cell cytotoxicity. It also provokes contraction of parachymal strips and stimulates microvascular changes. Recent findings indicate that LXA4 inhibits leukotcyte-dependent inflammation. Determination of LXA4 levels may provide new understanding of the role of LXA4 in basic cellular reactions and in pathophysiology of inflammation and other disease processes.This competitive ELISA allows for quantitative analysis of Lipoxin A4 levels in biological fluids. Product No.EA 45 |