|
||
|
|
Cell Biology and Cancer Research
|
Histamine EIA: This heterocyclic primary amine is derived form the decarboxylation of histidine. Histamine serves as a mediator of inflammation and hypersensitivity (anphylaxis) responses. Once sysnthesized by the histidine carboxylase enzyme , histamine is typically stored in metachromatic granules of basophils and mast cells (granulocytes) for later dispersion. Histamine is involved in a cascade physiological effects that are influenced by typical immune response and environmental factors. This assy, like many of our other assays, is developed for quantitative detection while providing both ease and convenience. The histamine assay is a competative ELISA that allows for competition between free histamine from the sample and HRP labeled histamine provided in the kit. A reaction with TMB substrate produces color developement that is inversely proportional to the amount of histamine present. This color can be read at 650 nm or quenched in acid solution to read at 450 nm. Product Number EA 31 Price: £Ü689,000 Instruction Booklet-Requires Adobe Acrobat |
|||||||||||||||
|
|
|||||||||||||||
|
Cross Reactivity
|
Contents
|
||||||||||||||
Products for Apoptosis Studies
|
Anti-Human bcl-2 (Sheep): Human hematopoetic malignancies are often characterized by gene rearrangemnts that deregulate gene expression. Antibodies to human bcl-2 (B cell leukemia/lymphoma 2) are directed against the gene product isolated from chromosomal breakpoint t (14;18) of bcl-2, which suppresses programmed cell death (apoptosis) and appears to be modulated by association with bax, a cell death promoter. Useful for Western blot immunopreciptation and immunoflourescence studies. Product Number: AP 63 |
|
Anti-Human bcl-2 (monoclonal): Overexpression of bcl-xl to other bcl-2 members is believed to promote cell survival. The ratio of bcl-xl to other family members is believed to modulate the apoptotic process. The immunogen used to produce this monoclonal to human (clone 2H12) was a synthetic peptide corresponding to animo acids 3 to 14 of the juman bcl-xl sequence. This IgG purified antibody also reacts with mouse and rat bcl-xl. Product Number: AP 64 |
|
EZ-Prime Human bax Oligonucleotide Primers: This oligonucleotide primer set is designed for specific PCR amplification of human bax message, giving an amplimer of 131 bp. One vial is sufficient for > 100 amplification reactions, and is provided with instructions and recommended experimental conditions. Product Number: EZ 41 |
Reagents for Oncogene Research
Recombinant Oncogene Products -Activation of cellular oncogenes may be directly associated with the development of neoplasia in laboratory animals and humans. Increased expression or structural alterations in these critical genes have been demonstrated to be associated with malignant transformations. Specific methods for the detection of oncogene induction, activation and/or transformation are of great interest. We will continue to expand our products in this area.
H-Ras, Wild Type, Recombinant Human: The Ras oncogenes encode four closely related 21 kDa proteins (H-Ras, K-Ras4A, K-Ras4B and N-Ras). The frequent detection of Ras oncogene products in human tumors, strongly implicate a role for these proteins in human tumorigenesis. Ras is a G protein which functions in GDP/GTP regulated signal transduction reactions, including the MAP kinase cascade which activates nuclear transcription factors. This recombinant Ras protein was produced in E. coli and is over 95% pure. It binds GDP and is a substrate for prenylation by farnesyl transferase. Product Number: CP 10
H-Ras, CVLL Variant, Recombinant Human: In contrast to wild-type Ras, which has a arboxy-terminal sequence of CVLS, this genetic variant terminates with the sequence CVLL and is a specific substrate for geranyl-geranyl transferase. Product Number: CP 12 |
cDNA probes for Oncogene ResearchcDNA Probe for c-myc: This large (1.8 kb) fragment of the c-myc oncogene is useful for the preparation, by nick translation or random priming, of labeled probes for quantitative analysis of c-myc expression by Northern blotting or in situ hybridization. Product Number: R 14 |
|
Recent studies indicate important roles for several subcellular organelles that maintain an acidic pH in the development of cellular response receptor-mediated endocytosis. DAMP: 3-(2,4-dinitroanilino)-3-amino-N-methyldipropylamine (DAMP), MW = 311.3, concentrates in acidic compartments of cultured cells, where it can be visualized after fixation and reaction with mouse monoclonal antibody directed against DNP (product D 07) followed by secondary antibody coupled to an enzyme or fluorescent dye. Useful for localization of organelles where receptor-ligand complexes are targeted, and identification of organelle functions induced by receptor-ligand interactions. Product Number: D 05 |
|
DAMP Kit: A convenient reagent kit designed for cytochemical visualization of acidic compartments. This method can be used in conjunction with light or electron microscopy and offers important advantages over the use of vital pH indicators The unique reagents required for this procedure are: 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine or DAMP (product D 05), a basic congener of dinitrophenol (DNP), and monoclonal anti-DNP (product D 07). The kit comes complete with instructions. Product Number: D 09 Product Specification-Requires Adobe Acrobat |
|
Anti-DNP Monoclonal: In addition to its use in conjunction with DAMP for detection of acidic granules (product D 05), this product can also be used for detection of a variety of sparse antigens using immunogold labeling. Detection of sparse antigens with monoclonal anti-DNP involves an antibody "sandwich" technique. Prior to preparation of the sample for electron microscopy, tissue or cells (acting as antigen), are incubated with the antibody. The secondary antibody has been coupled to dinitrophenol (DNP) by a straightforward process, so that it is now labeled with multiple DNP residues. The tissue is then processed for electron microscopy. The monoclonal anti-DNP (the tertiary antibody) is then applied to thin sections followed by protein A-gold labeling. This method has been used to detect the receptor for low-density lipoprotein on human fibroblasts, which had previously evaded detection by protein A-gold in ultra-thin cryosections. Product Number: D 07 |
Apolipoproteins
|
Apolipoproteins have long been known to be ligands for the low density lipoprotein (LDL) receptor. Apolipoprotein E (Apo E) also binds to the b-amyloid and tau receptors, and has been shown to be involved in nerve degeneration and regeneration. Recent studies have found an association of apolipoprotein E with the onset of Alzheimer's disease in late onset families.
Apolipoprotein E2, Recombinant Human: This 34 kDa protein has a pI of approximately 6.25, produced in a baculovirus/insect cell system, and purified by affinity chromatography. It competes with iodinated human LDL for binding to the human LDL receptor, and binds to b-amyloid peptides and tau protein in an overlay ligand blot assay. Self association may occur at high Apo E concentrations. Product Number: AL 02 |
|
Apolipoprotein E3, Recombinant Human: This 34 kDa protein is produced in a baculovirus/insect cell system, has a pI of approximately 6.35. As for Apo E2, it competes with iodinated human LDL for binding to the human LDL receptor, and binds to b-amyloid peptides and tau protein in an overlay ligand blot assay. Product Number: AL 03 |
|
Apolipoprotein E4, Recombinant Human: This 34 kDa Apo E isoform is also produced in a baculovirus/insect cell system, and has a pI of approximately 6.7. As for Apo E2 and Apo E3, it competes with iodinated human LDL for binding to the human LDL receptor, and binds to b-amyloid peptides and tau protein in an overlay ligand blot assay. Product Number: AL 04 |
cDNA Probes and Oligonucleotide Primers for Cytokines
|
IL2r alpha Subunit Probe: This 400 bp probe for the a or p55 (Tac) subunit, is involved in low affinity binding of IL-2, but apparently cannot function in signal transduction unless associated with another functional membrane component. Product Number: N 50 |
IL2r beta Subunit Probe: The beta subunit shows intermediate affinity for IL-2 by itself and, in combination with the a subunit, forms the high affinity IL-2 alpha-beta receptor complex. This p70 ( beta subunit) probe is 1.7 kb. Product Number: N 55 |
|
cDNA Probe for Interleukin-2: IL-2 is one of the best characterized cytokines. It plays a major role in the antigen-specific proliferation of T lymphocytes, and also has a strong influence on the development and function of other cell types including: thymocytes, B cells, and natural killer cells. The cDNA probe is 450 base pairs long. Product Number: N 60 |
|
EZ-Prime Human Interleukin-4 Oligonucleotide Primers: This oligonucleotide primer set is designed for specific PCR amplification of human IL-4 message, giving an amplimer of 143 bp. One vial is sufficient for > 100 amplification reactions. Instructions and recommended experimental conditions are provided. Product Number: EZ 44 |
|
EZ-Prime Human Interleukin-10 Oligonucleotide Primers: This oligonucleotide primer set is designed for specific PCR amplification of human IL-10 message, giving an amplimer of 344 bp. One vial is sufficient for > 100 amplification reactions. Instructions and recommended experimental conditions are provided. Product Number: EZ 45 |
|
EZ-Prime Human TNF alpha Oligonucleotide Primers: This oligo nucleotide primer set is designed for specific PCR amplification of human TNF alpha message, giving an amplimer of 389 bp. One vial is sufficient for > 100 amplification reactions. Instructions and recommended experimental conditions are provided. Product Number: EZ 46 |
|
EZ-Prime Human Interferon Gamma Oligonucleotide Primers: This oligonucleotide primer set is designed for specific PCR amplification of human IFN gamma message, giving an amplimer of 389 bp. One vial is sufficient for > 100 amplification reactions. Instructions and recommended experimental conditions are provided. Product Number: EZ 47 |
|
Cortisol EIA Kit: Cortisol is the most potent glucocorticoid produced and secreted by the adrenal cortex. Cortisol has numerous physiological and pharmacological actions, including its anti-inflammatory effects. Plasma cortisol levels are useful in the diagnosis of Cushing's syndrome, Addison's disease, adrenal tumors, prostate cancer and depression. Product Number EA65 Instruction Booklet-Requires Adobe Acrobat |
|
Cortcosterone EIA: This glucocorticoid is secreted by the adrenal gland in response to stress for mammalian species. Glucocorticoids also serve a function in the metabolism of fats, protein and carbohydrate and can be adminstered as an anti-inflamitory agent. Levels of both cortisol and corticosterone increase in mammalian species due to stressors. Only the corticosterone is the dominant glucocorticoid elevated in response to stress for both mice and rats as well as other non-human mammalian species. The actual stressors are not always indicative for the elevated levels of corticosterone but among them are climate changes, changes in environment, rapid temperature changes and changes in daily activity. This ELISA for was developed for the quantitation of corticosterone in relation to stressors on a test subject. This ELISA is specific for corticosterone with minimal cross reactivity from other key glucocorticoids. Measurement of cortcosterone is obtained with a competitive ELISA design. The corticosterone from the sample competes for a limited number of binding sites with the enzye conjugate. Readings can be taken at 650 nm or 450 nm with stop solution. Oxford provides a specially formulated extraction buffer that allows most sample types to be assayed directly. Product Number EA66 Instruction Booklet-Requires Adobe Acrobat |
|
Testosterone EIA Kit: Testosterone, the principle androgen, is synthesized in the testis, the ovary, and the adrenal cortex. Plasma testosterone levels are useful in investigating hypogonadism and hormone-replacement therapy in men, and as a marker in hyper-androgenism in women. Product Number EA78 Instruction Booklet-Requires Adobe Acrobat |
|
Progesterone Assay Kit: Progesterone is an important intermediate in steroid biosynthesis in all tissues that secrete steroid hormones. Progesterone is responsible for the progestational changes in the endometrium and the cyclic changes in the cervix and vagina. The plasma progesterone level is useful in investigating the leutal function and monitoring ovulation. This competitive ELISA allows for quantitative analysis of progesterone levels. Product Number EA74 Instruction Booklet-Requires Adobe Acrobat |
|
Estradiol Assay Kit: 17b-Estradiol, considered the true ovarian hormone in women, is among the most potent estrogen. 17b-Estradiol is responsible for the reproductive epithelia, breast growth, maturation of long bones, and secondary female sex characteristics. The 17b-Estradiol level in plasma is used in assaying for fertility, amenorrhea, and precocious puberty in girls. This competitive ELISA provides quantitative analysis of Estradiol levels in biological fluids. Product Number EA70 Instruction Booklet-Requires Adobe Acrobat |
|
Estriol EIA Kit: Measurement of the total estriol has been shown to be useful in monitoring fetal function. During pregnancy, estriol begins to appear in the maternal circulatory system as a result of synthesis by the feto-placental unit, in the ninth week of gestation. During the third trimester, estriol levels in serum and urine normally increase rapidly. Therefore, a low or declining estriol letel may be indicative of fetal distress. Product Number EA71 Instruction Booklet-Requires Adobe Acrobat |
|
Androstenedione EIA Kit: Androstenedione is synthesized in the adrenal gland. It is the primary precursor of testosterone in women. Therefore, in women, androstenedione contributes significantly to the level of androgenic activity, while in men this contribution is negligible. Quantitation of androstenedione may indicate the androgenic activity in women. Product Number EA68 Instruction Booklet-Requires Adobe Acrobat |