Prostaglandin E2 EIA Kit

Prostaglandin E2 (PGE2) is derived from PGH2 which in turn is synthesized from arachidonic acid, through the cyclooxygenase pathway. Many cell types, such as, epithelial cells, fibroblasts, and macrophages produce PGE2. PGE2 possesses vasoactivity, modulates immune functions, regulates sleep-wake cycle and exhibits many other activities. This competitive ELISA kit provides quantitative analysis of PGE2 levels in biological fluids based on the competition between the PGE2 present in the sample and PGE2-enzyme conjugate for a limited number of antibody binding sites

Product No.EA 02     Instruction Booklet

Prostaglandin F2 alpha EIA Kit

Prostaglandin F2 alplha (PGF2 alpha) is produced in various tissues, it contracts smooth muscle and has been used to induce labor. It also has a luteolytic effect and has been considered a luteolytic hormone. Usually, urine and tissue culture supernatant can be assayed directly following dilution with EIA buffer. PGF2 alpha can be extracted from plasma using Sep-Pak columns according to the protocol provided

Product No.EA 05     Instruction Booklet

6-Keto-Prostaglandin F1 alpha EIA Kit

6 Keto-Prostaglandin F1 alpha (6-keto-PGF1 alpha) is a stable hydrolyzed product of unstable PGI2 (prostacyclin) which is derived from PGH2, which in turn is synthesized from the arachidonic pathway. Through the arachidonic pathway, it is the major product produced by the endothelial cells, although it is also synthesized in other cell types such as macrophages. Prostacyclin inhibits platelet aggregation and induces vasodilation. Quantitation of prostacyclin production can be made by determining the level of 6-keto-PGF1 alpha. Urine and tissue culture supernatants can usually be assayed directly by diluting them with the EIA buffer provided. 6-keto-PGF1 apha can be extracted from plasma using Sep-Pak columns according to a protocol provided with the kit

Product No.EA 08     Instruction Booklet

11 beta-Prostaglandin F2 alpha EIA Kit

11 beta-PGF2 alpha is derived from PGD2 by the action of 11-keto-reductase, a potent bronchoconstrictor agent, and also causes hypertension. Plasma levels of 11 beta-PGF2 alpha are highly elevated in patients with systemic mastocytosis with an overproduction of PGD2. Usually, urine and tissue culture supernatant can be assayed directly following dilution with EIA buffer. 11 beta-PGF2 alpha can be extracted from plasma using Sep-Pak columns according to a protocol provided with the kit

Product No.EA 11     Instruction Booklet

13,14-Dihydro-15-Keto-Prostaglandin F2 alpha EIA Kit

13,14-Dihydro15-Keto-PGF2 alpha is derived from PGF2 alpha through the consecutive actions of 15-hydroxy prostaglandin dehydrogenase and prostaglandin 13-reductase. Quantitation of this compound in circulation can be a good index for PGF2 alpha synthesis in tissue. This is a competitive ELISA kit. Usually, urine and tissue culture supernatant can be assayed directly, whereas plasma should be extracted prior to analysis

Product No.EA 20     Instruction Booklet

Thromboxane B2 EIA Kit

TXB2 is a stable hydrolysis product of TXA2, which is derived from PGH2 by the action of thromboxane synthase. It is a major product following platelet aggregation induced by a variety of agents such as thrombin and collagen. This competitive ELISA permits quantitative analysis of TXB2 levels in biological fluids. Usually, urine and tissue culture supernatant can be assayed directly, whereas TXB2 should be extracted from plasma prior to analysis

Product No.EA 25     Instruction Booklet

11-Dehdydrothromboxane B2 EIA Kit

Measurement of 11-dehydro-TXB2, one of the major metabolites of TXB2, in plasma is an approach to the assessment of thromboxane formation in circulation. Usually, urine and tissue culture supernatants can be assayed directly using this competitive ELISA, whereas 11-dehydro-TXB2 should be extracted prior to analysis

Product No.EA 30     Instruction Booklet

Ovine PGHS-1

Our PGHS-1 is a homogeneous protein purified from ram seminal vesicles. It is extensively used for investigations of nonsteroidal anti-inflammatory agents in academic and pharmaceutical research laboratories. It is also used to investigate peroxidase-reducing substrate activity and to study activation of precarcinogens. Each preparation of PGHS-1 is accompanied by complete information on its specific activity and stability along with detailed instructions for cyclooxygenase assay and PGH2 generation. A convenient kit, complete with substrate, cofactors and buffer, is also available (product number PG 02).Purity : Greater than 90% by electrophoresis (typical lot). PGH2 metabolizing enzymes are not detectable. Specific activity : > 50,000 units/mg protein (typical lot). One unit is the amount of enzyme that triggers uptake of one nmol O2 per minute in conjunction with the conversion of arachidonic acid to PGG2 under standard conditions.Packaging: Shipped as a frozen suspension in 30 mM Tris buffer (pH 8.0) containing 0.1% Tween 20 and a preservative. Stable indefinitely at -70°C

Product No.PG 01

Anti-PGH Synthase (goat polyclonal)

This antiserum to ovine PGHS-1 holoprotein reacts with PGHS-1 (COX-1) from most mammalian species,including rodents. It also cross-reacts moderately with PGHS-2 in several systems. It is therefore very useful for determination of total PGHS expression by Western blots. Immunoglobulin type: Polyclonal goat IgG. Contents: 100 µl. A dilution of 1/1000 for Western blots or 1/100,000 for ELISA assays will yield a positive result with sheep PGH synthase.

Product No.PG 19

Preimmune goat serum

Polyclonal goat IgG for use with PG19

Product No.PG 19C

Anti-PGH Synthase-1 (rabbit polyclonal)

This antiserum to ovine PGHS-1 (COX-1) reacts with PGHS-1 from most mammalian species, except rodents. It does cross-react slightly with PGHS-2. It can be used to monitor PGHS expression by Western blots. Contents(per vial): 1 ml rabbit polyclonal IgG Ab. A dilution of 1/100 for Western blots or 1/10,000 for ELISA assays will yield a positive result with sheep PGH synthase

Product No.PG 20

Preimmune rabbit serum

Polyclonal rabbit IgG for use with PG 20

Product No.PG 20C

Polyclonal anti-PGHS-1 peptide

For studies that demand a polyclonal antibody with the highest specificity for PGHS-1, this antibody was prepared by immunizing rabbits with a synthetic peptide that corresponds to a sequence region unique to PGHS-1. This antibody is specific for PGHS-1 and reacts with native, as well as, recombinant PGHS-1 from several species including humans. However, it does not react with murine PGHS-1. It does not cross-react with PGHS-2 from any of the species examined to date. Each vial contains sufficient antibody to perform at least 5 Western blots

Product No.PG 16

Monoclonal Anti-PGH Synthase-1 (Anti-PGHS-1, Anti COX-1)

This monoclonal antibody was developed against native ovine PGHS-1 and reacts with PGHS-1 from sheep, bovine, human, and rat. It does not react with guinea pig, rabbit, mouse, or dog PGHS. Applications: Immunoassay or immunohistochemical localization of PGHS-1. Not suitable for Western blotting. Contents(per vial): 1 ml of sterile culture medium containing approximately 90 µg of monoclonal mouse IgG2b from the cyo-1 hybridoma cell line along with 0.1% sodium azide

Product No.PG 21

Monoclonal Anti-PGH Synthase-1 (anti-PGHS-1, anti COX-1)

This monoclonal antibody was developed against ovine PGHS-1 and reacts with PGHS-1 from sheep, bovine, guinea pig, rabbit, and human. This monoclonal does not react with rat, mouse, or dog PGH synthase. Applications: Immunoassay or immunohistochemical localization of PGHS-1. Not suitable for Western blotting. Contents(per vial): 1 ml of sterile culture medium containing approximately 50 µg of monoclonal mouse IgG2b from the cyo-5 hybridoma cell line along with 0.1% sodium azide

Product No.PG 22

Anti-PGH Synthase-1 (Anti-PGHS-1, Anti-COX-1) Monoclonal

This monoclonal antibody was developed against denatured ovine PGHS-1 and does not cross react with PGHS-2. It is useful for specific detection of COX-1 on Western blots or other immunoassays. Each vial contains sufficient antibody to perform at least 5 Western blots. Suggested dilution 1:1000.

Product No.PG 05

Prostaglandin H-Synthase-1 Western Blotting Standard

This highly purified native ovine COX-1 preparation is enzymatically inactive and very useful as a standard for Western blotting. Each vial is sufficient for at least 10 Western blots

Product No.NP 01

cDNA Probes For Prostaglandin H Synthase-1

The regulation of prostaglandin H synthase gene expression is a very active area of investigation. Translation of PGHS-1 and PGHS-2 mRNA appears to be very complex, and is also stimulated by growth factors and inhibited by steroids. At high stringency, the following probes specifically hybridize to the 3 kilobase mRNA that encodes prostaglandin H synthase. NOTE: At low stringency, they may also hybridize to the 4.2 kb mRNA that encodes PGHS-2

Product No. Ovine  R 74  / Murine R 75  / Human R 76

EZ-Prime Human COX-1 Primers

This oligonucleotide primer set is designed for specific PCR amplification of human or mouse prostaglandin H synthase form-1 (COX-1) message, giving an amplimer of 756 bp for either species. One vial is sufficient for > 100 amplification reactions, and is provided with complete instructions and optimal experimental conditions

Product No.EZ 01

Murine PGHS-1 (COX-1) Promoter Construct

This plasmid contains 2.3 kb of the 5'-flanking region of the murine PGHS-1 linked to a luciferase reporter gene. Upon transfection into a murine cell line, this construct can be used to investigate PGHS-1 gene regulation

Product No.PG 07

Prostaglandin H-synthase-2 ( COX-2), recombinant human

We have expressed recombinant human cyclooxygenase-2 in insect cells from a baculoviral vector. This partially purified preparation is useful for rapid screening of novel COX-2 specific inhibitors. Each vial is sufficient for up to 100 radiochemical enzyme assays, and complete with an easy protocol that even a relative novice can follow. This enzyme can also be used as a standard for Western blotting. Contents: At least 100 units of PGHS-2 enzyme in 500µl in 5mM Tris pH 8.0 containing 1µg/ml each of pepstatin and leupeptin and 1 mM pefablock SC 4-(2-amino-ethyl) benzene sulfonyl flouride. Source: Insect cell lysate supernatant Specific activity: 400 units/mg protein (typical lot) Definition of unit: One unit is the amount of enzyme that exhibits a velocity of one nanomole of O2 per minute in conjunction with the conversion of arachidonic acid to PGH2 at 37°C under assay conditions described in the instructions. Storage: Stable indefinitly at -70°C

Product No.RP 02

Anti-PGHS-2 (C-terminus), Polyclonal

This antibody was generated against a synthetic peptide corresponding to a distinct C-terminal region of murine PGHS-2. It specifically reacts with PGHS-2 from most species thus far examined. It does not cross-react with PGHS-1. Contents(per vial): 1 ml. A dilution of 1/100 for Western blots or 1/100,000 for ELISA assays will yield a positive result with native ovine or recombinant human PGHS-2

Product No.PG 26

Preimmune Control Serum

For experiments using PG 26.

Product No.PG 26C

Polyclonal Rabbit Anti-Human PGHS-2

This antibody was generated against a synthetic peptide corresponding to a distinct C-terminal region of human PGHS-2. It specifically reacts with PGHS-2 from most species thus far examined. It does not cross-react with PGHS-1.: 1 ml. A dilution of 1/100 for Western blots or 1/100,000 for ELISA assays will yield a positive result with recombinant human PGHS-2

Product No.PG 27

Polyclonal Rabbit Anti-Human PGHS-2

This antibody was generated against a synthetic peptide corresponding to a distinct C-terminal region of human PGHS-2, as was the antibody described above. It specifically reacts with PGHS-2 from most species thus far examined. It does not cross-react with PGHS-1. It has been widely used for immunohistochemical studies of human PGHS-2 expression

Product No.PG 27b

Preimmune Control Serum

For experiments using PG 27b

Product No.PG 27C

Anti-PGH Synthase-2 (Anti-PGHS-2, Anti-COX-2) Monoclonal

This monoclonal antibody, developed against a peptide corresponding to a unique sequence of human COX-2, does not cross react with PGHS-1. It is useful for specific detection of COX-2 on Western blots using enhanced chemiluminescence. Each vial contains sufficient antibody to perform at least 5 Western blots

Product No.PG 28

Prostaglandin H-Synthase-2 (Cyclooxygenase-2), Ovine

This purified COX-2 isoform from ovine placenta is not enzymatically active. However, it is very useful as a standard for Western blotting or other immunochemical procedures. Simply mix with sample buffer, boil, and run on SDS gel. For best results, use 1µg per lane when developing with alkaline phosphatase. Contents: 10 µg of protein in 100 µl.Protein Concentration: 0.1 mg/ml. Purity: Greater than 70% by electrophoresis. Packaging: The enzyme is suspended in 100 mM Tris buffer (pH 8.0) containing 0.01% Tween 20 and 30 µM DDC. Storage: Stable for several months at -20°C, and indefinitely -70°C

Product No.NP 04

cDNA Probes For Prostaglandin Synthase-2

COX-2 has been cloned from various species. At high stringency, these probes specifically hybridize to the 4.2 kb mRNA that encodes PGHS-2. NOTE: Under low stringency conditions, probes for the PGHS-1 gene (see above) also hybridize with COX-2 mRNA, but not under high stringency.

Product No.  R 77 chicken  / R 78 murine  / R 91 human / R 92 rat

EZ-Prime Human COX-2 Primers

EZ-Prime Human COX-2 Primers: This oligonucleotide primer set, designed for specific PCR amplification of human or mouse prostaglandin H synthase form-2 (COX-2) message, gives an amplimer of 724 bp for either species. One vial is sufficient for > 100 amplification reactions, and is provided with complete instructions and optimal experimental conditions.

Product No.EZ 02

Murine PGHS-2 Promoter Construct (Short)

This plasmid contains 960 bp of the 5'-flanking region of the murine PGHS-2 linked to a luciferase reporter gene. Upon transfection into a murine cell line, this construct can be used to investigate PGHS-2 gene regulation

Product No.PG 06

Murine PGHS-2 Promoter Construct (Long)

This plasmid contains 3.3 kb of the 5'-flanking region of the murine PGHS-2 linked to a luciferase reporter gene. Upon transfection into a murine cell line, this construct can be used to investigate PGHS-2 gene regulation

Product No.PG 09

Ibuprofen

This is a selective prostaglandin H synthase inhibitor with an IC50 = 14.9 µM for COX-1. MW = 206.3, Purity: 99%.

Product No.EB 10

Piroxicam

Prostaglandin synthase inhibitor. MW = 331.3, Purity: 99%.

Product No.EB 14

Tranylcypromine-HCl

Functions to inhibit Cox-2 without the inhibiton of Cox-1. MW = 314.4 Purity: 98%

Product No.BP 10

Indomethacin

This selective prostaglandin synthase inhibitor, IC50 = 0.1 µM for COX-1, also inhibits lipoxygenases but with an IC50 > 100 µM. MW = 357.8, Purity: 99%.

Product No.EB 12

NS-398

Functions to inhibit COX-2 with no Inhibition of COX-1. MW = 314.4, Purity: 98%

Product No.EB 15

Anti-PGD Synthase (Hematopoietic Form) Polyclonal

This antibody was prepared in rabbit using S-hexylglutathion affinity column purified rat hematopoietic form PGD Synthase as the immunogen. It is specific for hematopoietic form PGD Synthase, and does not cross react with brain form of PGD Synthase. Each vial contains 100 µL antibody. This antibody is useful for enhanced chemiluminescence, immunohistochemistry, immunoprecipitation and ELISA applications. For Western blots using alkaline phosphatase, a dilution of 1: 800 is recommended.

Product No.PD 01

cDNA Probe for PGD Synthase (Hematopoietic Form)

This 482 bp fragment of the cDNA encoding hematopoietic form PGD synthase is useful for the preparation, by nick translation or random priming, of labeled probes for Northern blot analysis of hematopoietic PGD synthase expression.

Product No.PD 03

cDNA Probe for Brain Form PGD Synthase

This fragment of the cDNA encoding the brain isoform of PGD synthase (b-trace protein) is useful for the preparation, by nick translation or random priming, of labeled probes for Northern blot analysis of the expression of the brain isoform of PGD synthase

Product No.PD 04

EZ Prime PGD Synthase Primers

This 17 mer oligonuceotide primer set is designed for specific PCR amplification of hematopoietic form PGD synthase message, and gives an amplimer of 482 bp. Each vial is sufficient for 20 amplification reactions and provides instructions and optimal reaction conditions (100 µL/vial with 100 µM concentration)

Product No.EZ 30

Anti-Prostacyclin Synthase (Monoclonal)

This antibody reacts with prostacyclin synthase from most mammals, including; bovine, mouse, rat, sheep, guinea pig and rabbit. It can be used for immunoassay, immunohistochemistry and immunoaffinity purification. Contents (per vial): 1 ml of culture medium from the isn-1 hybridoma cell line containing >20 µg of mouse IgG1 in 0.1% sodium azide.

Product No.PG 23

Prostacyclin Synthase, Ovine

This solubilized and partially purified preparation of mammalian prostacyclin synthase should therefore facilitate basic and pharmaceutical research, including study of the factors involved in the regulation of prostacyclin synthase gene expression, development of novel therapeutic agents for the treatment of cardiovascular diseases and development of new strategies for the prevention and management of thromboembolic episodes. Also useful as a standard in Western blotting experiments.

Product No.PG 11

cDNA Probe for Prostacyclin Synthase

This fragment of the cDNA encoding human prostacyclin synthase is useful for the preparation, by nick translation or random priming, of labeled probes for Northern blot analysis of the expression of the brain isoform of prostacyclin synthase

Product No.R 80

EZ-Prime Prostacyclin Synthase Primers

This oligonuceotide primer set is designed for specific PCR amplification of human prostacyclin synthase message, giving an amplimer of 544 bp. One vial is sufficient for >50 amplification reactions, and is provided with complete instructions and optimal experimental conditions.

Product No.EZ 11

Anti-Prostaglandin E2

Polyclonal rabbit antiserum diluted in phosphate buffered saline with 0.1% sodium azide. Each vial contains 1 ml, sufficient for >100 tubes in RIA. Sensitivity: 130 picograms of unlabeled PGE2 will result in 50% inhibition of binding of radiolabeled ligand il a standard liqtid phase radioimmunoassay system.

Product No.PG 31

Anti-Thromboxane B2

A highly sensitive and specific monoclonal antibody for quantitation of TxB2 in tissue extracts, serum, etc. Sensitivity: 20 pg TxB2/mL. Sufficient for at least 100 assays. Cross-reactivity, negligible with other eicosanoids

Product No.TB 01

Anti-Thromboxane B2, Sheep Polyclonal

Each vial of this antibody is sufficient for 100 tubes in a standard RIA protocol. Cross-reactivity : PGF2 alpha (0.13%), and 6-keto-PGE1 (0.17%). Negligible (<0.07%) cross-reactivity with other eicosanoids.

Product No.TB 04

Anti-PGF2 alpha, Sheep Polyclonal

Each vial of this antibody is sufficient for 100 tubes in a standard RIA protocol. Cross-reactive with PGF1 alpha (21%) and TXB2 (16.0%). Negligible cross-reactivity with other eicosanoids.

Product No.PG 32

Anti-6-keto-PGF1 alpha, Sheep Polyclonal

Each vial of this antibody is suffichent for 100 tubds in a standard RIA protocol. Cross-reactive with: 6-keto-PGE1 (8.6%), PGF1 alpha (7.9%), and PGF2 alpha (2.1%). Negligible (<0.07%) cross-reactivity with other eicosanoids.

Product No.PG 40