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Ecotin: Ecotin
is a serine protease inhibitor that is found in the periplasm of Escherichia
coli. It has a very broad range of inhibition including the pancreatic serine
proteases trypsin and chymotrypsin, human leukocyte and porcine elastase and rat
mast cell chymase. Ecotin is regarded as a potent, tight-binding, reversible
inhibitor of Factor Xa. Ecotin does not inhibit the human plasma proteases
thrombin, tissue factor-factor VIIa, Factor XIa, Protein C, plasmin or
tPA.
Product No.: RA11 Qty. : 1.0 mg
Neutrophil Elastases: Neutrophils phagocytize or release bacteriostatic agent as a
function of immune defense in the presence of foreign particles. Serine protease
neutrophil elastase is stored in high abundance contained in azurphlic granules
of the neutrophil and act to digest the phagocytized foreign partilcles. See
product specificaiton sheet for individual information.
|
Product No. |
Human SP 21 |
Mouse SP 25 |
Rat SP 27 |
Porcine SP 23 |
|
Qty |
0.5 mg |
0.1 mg |
0.1 mg |
0.1 mg |
Immobilized Porcine Pancreatic Elastase:
This immobilized elastase is useful in cleaving
substrate proteins such as PAI-1. The immobilized elastase cleaved PAI-1 between
the P3 and P4 residues creating a loop-inserted species that is not inhibitory
to uPA and tPA.
Product No.: SP 31 Qty.: 1.0 mg
I
mmobilized Soybean Trypsin
Inhibitor(SBTI): SBTI is acts as a reversable
competitive inhibitor of trypsin an proteases such as chymotrypsin, plasmin and
plasma kallikren.
Product No.: SP 29 Qty.: 10 ml
Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found
in plasma that is implicated in the processes of angoigenisis, hemostasis, tumor
metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1).
The elevation of PAI-1 has shown to correlate with the prognosis of many cancers
including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein
vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell
migration (5). This binding has proven to be an important inhibitor of the
activated form of protein C (APC) (6).
PAI-1 is a member of the SERine
Protease INhibitor superfamily (SERPINS) and will spontaneously decay to the
latent or inactive form. This inactive form has a half-life of approximately 1
hour at physiogical pH and temperature. The binding of vitronectin also serves
to stabilize PAI-1, nearly doubling its half-life at physiological pH and
temperature.
PAI-1 is also found to interact with low-density
lipoproteins (LDL) including LDL receptor related protein (LRP), very
low-density lipoprotein (VLDL) and gp330/megalin
(7).
PAI-1 (active wild type): This PAI-1 is produced in E. coli and purified gently to provide
an active fraction. Each product is > 99% pure and >98%
active.
|
Product No. |
Human PI01 |
Mouse PI16 |
Rat PI19 |
Rabbit PI23 |
Porcine PI22 |
|
Qty. |
0.5 mg |
0.5 mg |
0.5 mg |
0.5 mg |
0.5 mg |
PAI-1 (latent wild type): This latent form of PAI-1 is < 0.4% active and useful for
control for experiments when used in conjunction with the active form of
PAI-1.
|
Product No. |
Human PI 02 |
Mouse PI 17 |
Rat PI 20 |
Rabbit PI 24 |
|
Qty |
0.5 mg |
0.5 mg |
0.5 mg |
0.5 mg |
PAI-1 (substrate form/ P12 Glu):
This PAI-1 has a single substitution at
position P12 in the reactive center loop fascilitating a conversion to a
substrate for proteinases rather than an inhibitor. Useful for mechanistic
studies.
Product No.: Human PI 03 Qty.: 0.5 mg
PAI-1 (substrate form/ P14 Glu):
This PAI-1 has a single substitution at position
P14 in the reactive center loop fascilitating a conversion to a substrate for
proteinases rather than an inhibitor. Useful for mechanistic
studies.
Product No.: Human PI 04 Qty.: 0.5 mg
PAI-1 (stable mutant): This PAI-1 is a collection of four mutations: K154T, Q139L, M354I
and H150H. These mutations offer great stability while locking into the active
conformaton. Ideal for in vivo studies.
|
Product No. |
Human PI 05 |
Mouse PI 18 |
Rat PI 21 |
|
Qty |
0.5 mg |
0.5 mg |
0.5 mg |
Human PAI-1 (stable mutant/ N-terminal
poly- Histidine tag): This Human PAI-1 is a
collection of four mutations: K154T, Q139L, M354I and H150H. These mutations
offer great stability while locking into the active conformaton. The N-terminuse
has been additionally modified with the coupling of a 6-X His tag. The tag
allows for the Ideal for in vivo studies.
Product No.: Human PI 06 Qty.: 0.5 mg
Human PAI-1 (N-terminal Cys mutant/ active
fraction): This PAI-1 is a cloned wild type
active fraction with a cysteine residue coulpled to the N-terminnus. The
fraction is > 95% pure and > 98% active. Supplied in buffer with 1 mM DTT
to prevent dimerization.
Product No.: Human PI 80 Qty.: 0.5 mg
Human PAI-1 (N-terminal Cys mutant/ latent
fraction): This PAI-1 is a cloned wild type
latent fraction with a cysteine residue coulpled to the N-terminnus. The
fraction is > 95% pure and < 0.4% active. Supplied in buffer with 1 mM DTT
to prevent dimerization.
Product No.: Human PI 080 Qty.: 0.5 mg
Human PAI-1 (N-terminal Cys mutant/ stable
mutant): This PAI-1 is a stable fraction with a
cysteine residue coulpled to the N-terminnus. Supplied in buffer with 1 mM DTT
to prevent dimerization.
Product No.: Human PI 82 Qty.: 0.5 mg
Human PAI-1 (N-terminal biotin labled/
active fraction): This PAI-1 is a cloned wild
type active fraction with a biotin labeled substitution at the N-terminal
cysteine. The fraction is > 95% pure and > 98% active..
Product No.: Human PI 83 Qty.: 0.5 mg
Human PAI-1 (N-terminal biotin labled/
latent fraction): This PAI-1 is a cloned wild
type latent fraction with a biotin labeled substitution at the N-terminal
cysteine. The fraction is > 95% pure and < 0.4% active.
Product No.: Human PI 84 Qty.: 0.5 mg
Human PAI-1 (N-terminal biotin labeled/
stable mutant): This PAI-1 is a stable fraction
with a biotin labeled substitution at the N-terminal cysteine.
Product No.: Human PI 85 Qty.: 0.5 mg
Human PAI-1 (N-terminal FITC labeled/
stable mutant): This PAI-1 is a stable fraction
with a biotin labeled substitution at the N-terminal cysteine.
Product No.: Human PI 86 Qty.: 0.5 mg
Human PAI-1 (P9-NBD/ Ser338):
The P9-NBD PAI-1 is created via mutagenesis of
the P-9 serine residue (Ser338) on the react center loop to cysteine. This
promoted the availabilty of a free thiol group for incorporation of
N,N'-dimethyl-N- (acetyl)-N'- (7-nitrobenz- 2-oxa-1,3- diazol-4-yl) (NBD), a
fluorescent probe highly sensitive to changes in solvation and hydrophobic
environment. The fluorescence emission of P9-NBD PAI-1 is enhanced 6-7 fold upon
insertion of the reactive center loop into beta-sheet A following complex
formation with proteinases, formation of the latent species, or cleavage by
elastase. The incorporated probe is excited at 480 nm and displays a broad
emission spectrum with a peak centered 542 nm with a resultant blue-shift to 520
nm following reactive center loop insertion. The modified PAI-1 is nearly as
active as wt PAI-1 and is more resistant to the spontaneous latency reaction
making this an excellent tool for monitoring reaction rates of PAI-1 (1). P9-NBD
PAI-1 has been utilized in a number of studies to determine the rates of loop
insertion and SERPIN reaction mechanisms when reacted with various proteinases
(1,2), inactivating antibodies (3) and conformational changes imposed by the
binding of vitronectin (4).
Product No.: Human PI 08 Qty.: 0.5 mg
Human PAI-1 (P9-NBD/ Ser347):
The Human PAI-1 NBD labeled at the scissile bond
of the reactive loop P1'-NBD PAI-1 was created by mutagenesis of the P1' serine
residue (Ser347) at the P1-P1' scissile bond to cysteine. This provided a free
thiol group for NBD labeling, a fluorescent probe highly sensitive to changes in
solvation and hydrophobic environment. The fluorescence emission of P1'-NBD
PAI-1 is quenched upon cleavage of the reactive center loop by a target
proteinase.
Product No.: Human PI 09 Qty.: 0.5 mg
Human PAI-1 (elastase cleaved/ wild type latent): This PAI-1 is cleaved the the P3-P4 residues via immobilized elastase. Useful as a control for non-reactive and reactive loop inseterted species of PAI-1. < 1% active.
Product No.: Human PI 13 Qty.: 0.5 mg
Human PAI-1 (non-vitronectin binding
mutant): A single mutation of this PAI-1 mutant
prevents the binding to the ligand vitronectin. All other functions and
characteristics remain unchanged.
Product No.: Human PI 07 Qty.: 0.5 mg
Human PAI-1 (minimal vitronectin binding
latent): This PAI-1 demonstrates minimal
vitronectin binding in the latent form as provided.
Product No.: Human PVI 07 Qty.: inquire
Human PAI-1 (Ser-Cys/ NBD labeled):
This PAI-1 mutant has a protein replacement of
Ser to Cys at the vitronectin binding site. A NBD label is incorportated for
detection of vitronectin binding.
Product No.: Human PI 10 Qty.: 0.5 mg
Human PAI-1/ Latent Control (active mutant/
vitronectin binding): This control protein is
useful in reporting vitronectin binding.
Product No.: Human PV 10 Qty.: inquire
Octapeptide TVASSSTA activator (PAI-1
activation peptide): The octapeptide sequence
(TVASSSTA) mimmics the N-terminal residues of the reactive center loop of PAI-1
(cf. P14-P7 residues). This substituion of strand 4 in beta-pleated sheet A
competes with the formation of the latent species. This insertion then converts
PAI-1 into a substrate for tissue-type plasminogen activator (tPA) and other
target proteinases. The result is a thermodynamically stable PAI-1 species that
is useful for investigating the role of reactive center loop insertion. Supplied
as a lyophilized powder.
Product No.: Human PI 14 Qty.: 0.5 mg
Octapeptide TVASSSTA Pre-Annealed PAI-1
(P9-NBD/ Ser347): Active wild-type PAI-1
pre-annealed with the Octapeptide TVASSSTA and ready for immediate use. Please
inquire regarding other species of annealed PAI-1.
Product No.: Human PI 15 Qty.: 0.5 mg
PAI-1 Inactivating Peptide (TVASSSTA-OH):
This protein is an octapeptide mimic that binds
to the reactive center loop effectively competing with PAI-1. This protein is
useful in reactive center loop studies.
Product No.: Human IP 10 Qty.: 0.5 mg
Human PAI-1 Depleted Plasma (Na Citrate):
These preparations were made from frozen plasmas
using immobilized anti-PAI-1 IgG.
|
Product No. |
Anti-Human PI 50 |
Anti-Mouse PI 51 |
Anti-Rat PI 52 |
Anti-Rabbit PI 53 |
|
Qty |
10 ml |
10 ml |
10 ml |
10 ml |
Plasminogen, a single chain glycoprotein zymogen, is the precursor
the fibrinolytic enzyme plasmin. The native form of plasminogen is composed of
791 amino acids with glutamic acid located at the N-terminal portion
(Glu-plasminogen). Plasminogen has five homologous regions known as kringles.
These regions are specific for the complimenting kringles located on tPA, uPA
and prothombin. These krigles have one high affinity and four low affinity
lysine binding sites that mediate the interactions of plasminogen with fibrin
and alpha-2-antiplasm.
Plasminogen is converted to plasmin via a cascade
of various reactions that result in the hydrolysis of Arg560-Val561 peptide bond
of plasminogen resulting in two chains that remain covalently associated by a
disulfide bond.
Substrates:
Glu Plasminogen: These plasminogens were prepared from fresh plasma by lysine
sepharose chromatography.
|
Hu PL20 |
Mo PL28 |
Rat PL32 |
Rab PL36 |
Bov PL24 |
Ch PL40 |
|
10. mg |
10. mg |
10. mg |
10. mg |
10. mg |
10. mg |
Lys Plasmin: These plasmins were prepared from Glu Plasminogens by activation with immobilized uPA.
|
Hu PL22 |
Mo PL30 |
Rat PL34 |
Rab PL38 |
Bov PL26 |
Ch PL42 |
|
10. mg |
10. mg |
10. mg |
10. mg |
10. mg |
10. mg |
Antibodies:
Anit-Plasminogen (antiserum):
This polyclonal antibody is specific for
plasminogen and is a useful tool in fibrnolytic and cancer studies. Suitable for
use in western blot and ELISA applications. IHC not
determined.
Product No.: Anti-Mouse PL 60 Qty.: 1.0 mg
Anti-Plasminogen (IgG fraction):
This polyclonal antibody is specific for
plasminogen, purified and provided as an IgG fraction. Suitable for use in
western blot and ELISA.
Product No.: Anti-Mouse PL 62 Qty.: 1.0 mg
Anti-Plasminogen (IgG fraction/ Biotin
labled): This IgG fraction is labled with Biotin.
Suitable for use in western blot and ELISA. IHC not
determined.
Product No.: Anti-Mouse PL 66 Qty.: 0.5 mg
Anti-Plasminogen (IgG fraction/ FITC
labled): This IgG fraction is labled with
fluorescein. Suitable for use in western blot and ELISA. IHC not
determined.
Product No.: Anti-Mouse PL 65 Qty.: 0.5 mg
Anti-Plasminogen (IgG fraction/ high
titer): This polyclonal antibody is affinity
purified with IgG high titer (greater than 1x10^6) immobilized plasmin. Suitable
for use in western blot, IHC and ELISA.
Product No.: Anti-Mouse PL 64 Qty.: 1.0 mg
Antiplasmin Antibody (IgG fraction):
This purified rabbit polyconal antibody is to
mouse antiplasmin. Suitable for use in western blot. IHC not
determined.
Product No.: Anti-Mouse PL 49 Qty.: 1.0 mg
Non-Inhibitory Antiplasmin Antibody:
This purified monoclonal antibody is produced in
an antiplasmin knockout mouse. Suitable for use in western blot. IHC not
determined.
Product No.: Anti-Mouse PL 55 Qty.: 1.0 mg
Inhibitory Antiplasmin Antibody:
This purified monoclonal antibody is produced in
an antiplasmin knockout mouse. Suitable for use in western blot. IHC not
determined.
Product No.: Anti-Mouse PL 56 Qty.: 1.0 mg
Inhibitory Antiplasmin Antibody:
This purified monoclonal antibody is produced in
an antiplasmin knockout mouse. Suitable for use in western blot. IHC not
determined.
Product No.: Anti-Mouse PL 57 Qty.: 1.0 mg
Human tPA (single chain): This tPA is single chain and greater than 85% pure.
Product No.: Human PA 51 Qty.: 0.1 mg
Human tPA (single chain/ FITC label):
This tPA is single chain labled with fluorescein
and greater than 85% pure. Completely complexable with human
PAI-1.
Product No.: Human PA 52 Qty.: 0.1 mg
Human tPA (single chain/ texas red): This tPA is single chain labled with texas red and greater than 85% pure. Completely complexable with human PAI-1
Product No.: Human PA 53 Qty.: 0.1 mg
Human tPA (double chain): This tPA is double chain activated from the single-chain form and greater than 95% pure. Completely complexable with human PAI-1
Product No.: Human PA 54 Qty.: 0.1 mg
Recombinant Mouse tPA (active):
This recombinant mouse active tPA is produced in
insect cells using baculovirus.
Product No.: Human PA 56 Qty.: 10 ug
Tissue Plasminogen Activator (tPA) is tissue specific for activation of plasminogen. The kingle-2 domain of tPA reacts with lysine residues on the fibrin forming an activated complex. tPA is a single chain molecule that is cleaved to the active two-chain form by the action of the plamin pathway. tPA vastly exists coupled to PAI-1 in ciculation.
Anit-tPA (antiserum): This polyclonal antibody is specific for tPA and is a useful tool
in fibrnolytic and cancer studies. Suitable for use in western blot, IHC and
ELISA applications.
Product No.: Anti-Human PA 57 Qty.: 1.0 ml
Anti-tPA (IgG fraction): This polyclonal antibody is specific for tPA, purified and
provided as an IgG fraction. Suitable for use in western blot, IHC and
ELISA.
Product No.: Anti-Human PA 59 Qty.: 1.0 mg
Anti-tPA (IgG fraction/ Biotin labled):
This IgG fraction is labled with Biotin. Suitable
for use in western blot, IHC and ELISA.
Product No.: Anti-Human PA 33 Qty.: 1.0 mg
Anti-tPA (IgG fraction/ FITC labled):
This IgG fraction is labled with fluorescein.
Suitable for use in western blot, IHC and ELISA.
Product No.: Anti-Human PA 34 Qty.: 1.0 mg
Anti-tPA (IgG fraction/ high titer):
This polyclonal antibody is affinity purified
with IgG high titer (greater than 1x10^6) immobilized tPA. Suitable for use in
western blot, IHC and ELISA.
Product No.: Anti-Human PA 35 Qty.: 0.1 mg
Mouse Monoclonal Anti-tPA: This purified monoclonal antibody is to human tPA and binds well
to both the free and complexed form of tPA. Suitable for use in western blot and
ELISA.
Product No.: Anti-Human PA 64 Qty.: 1.0 mg
Mouse Monoclonal Anti-tPA (clone 1):
This purified monoclonal antibody is to human tPA
clone 1 and non-competitive with human tPA clone 2. Suitable for use in western
blot. IHC not determined.
Product No.: Anti-Human PA 6 Qty.: 1.0 mg
Mouse Monoclonal Anti-tPA (clone 2):
This purified monoclonal antibody is to human tPA
clone 2 and non-competitive with human tPA clone 1. Suitable for use in western
blot. IHC not determined.
Product No.: Anti-Human PA 61 Qty.: 1.0 mg
Non-Inhibitory Mouse Monoclonal Anti-tPA:
This purified capture monoclonal antibody is
produced in a tPA knockout mouse. Suitable for use in western blot and ELISA.
IHC not determined.
Product No.: Anti-Human PA 62 Qty.: 1.0 mg
Non-Inhibitory Mouse Monoclonal Anti-tPA:
This purified monoclonal antibody is produced in
a tPA knockout mouse. Suitable for use in western blot and ELISA. IHC not
determined
Product No.: Anti-Human PA 63 Qty.: 1.0 mg
Urinary-type plasminogen activator (uPA) reacts with many
synthetic substrates but the only physiological substrate is plasminogen. uPA¡¯s
role in fibrinolysis is to convert plasminogen to the active form plasmin. uPA
is inhibited by the presence of PAI-1 which is responsible for the regulation of
fibrinolysis.
The uPA receptor (uPAR) enhances the efficiency of plasmin
activation and therefore promotes tumor metastasis and invasion. The uPA is
furthermore involved in the proteolytic cascade of matrix metalloproteinases
(NMP) that degrade collagen.
Anit-uPA (antiserum): This polyclonal antibody is specific for uPA and is a useful tool
in fibrnolytic and cancer studies. Suitable for use in western blot and ELISA
applications. IHC not determined.
Product No.: Anti-Human PA 65 Qty.: 1.0 ml
Anti-uPA (IgG fraction): This polyclonal antibody is specific for uPA, purified and
provided as an IgG fraction. Suitable for use in western blot and ELISA. IHC not
determined.
Product No.: Anti-Human PA 66 Qty.: 1.0 mg
Anti-uPA (IgG fraction/ Biotin labled):
This IgG fraction is labled with Biotin. Suitable
for use in western blot, IHC and ELISA.
Product No.: Anti-Human PA 67 Qty.: 1.0 mg
Anti-uPA (IgG fraction/ FITC labled):
This IgG fraction is labled with fluorescein.
Suitable for use in western blot, IHC and ELISA.
Product No.: Anti-Human PA 68 Qty.: 1.0 mg
Anti-uPA (IgG fraction/ high titer):
This polyclonal antibody is affinity purified
with IgG high titer (greater than 1x10^6) immobilized uPA. Suitable for use in
western blot, IHC and ELISA.
Product No.: Anti-Human PA 69 Qty.: 0.1 mg
Anti-uPA Receptor (IgG fraction):
This polyclonal antibody is specific for the uPA
receptor, purified and provided as an IgG fraction. Suitable for use in western
blot and ELISA. IHC not determined.
Product No.: Anti-Human PA 75 Qty.: 1.0 mg
Mouse Monoclonal Anti-uPA (capture):
This purified monoclonal antibody is produced in
a uPA knockout mouse. Suitable for use in western blot and ELISA. IHC not
determined
Product No.: Anti-Mouse PA 70 Qty.: 1.0 mg
Mouse Monoclonal Anti-uPA (detection):
This purified monoclonal antibody is produced in
a uPA knockout mouse and labeled with biotin. Suitable for use in western blot,
IHC and ELISA.
Product No.: Anti-Mouse PA 61 Qty.: 1.0 mg
HMW Urokinase: This high molecular weight uPA is derived from urine and supplied
as the two-chain form. This product is useful for plasminogen activation.
Product No.: Human PA 80
Qty.: 0.1 mg
HMW Urokinase (HRP conjugated):
This high molecular weight uPA is derived from
urine and supplied as the HRP-conjugated two-chain form.
Product No.: Human PA 83 Qty.: 0.1 mg
HMW Urokinase (FITC lable):
This high molecular weight uPA is derived from
urine and supplied as the two-chain form. This product has been labled with FITC
fluoresceins.
Product No.: Human PA 81 Qty.: 0.1 mg
LMW Urokinase: This low molecular weight uPA is derived from urine and supplied
as the single-chain form. This product is useful for plasminogen activation.
Product No.: Human PA 85 Qty.: 0.1 mg
LMW Urokinase (HRP conjugated):
This low molecular weight uPA is derived from
urine and supplied as the single-chain form. This uPA have been conjugated with
HRP for yourease and convenience.
Product No.: Human PA 86 Qty.: 0.1 mg
LMW Urokinase (FITC lable):
This low molecular weight uPA is derived from
urine and supplied as the single-chain form. This product has been labled with
FITC fluoresceins.
Product No.: Human PA 87 Qty.: 0.1 mg
Mouse uPA (kidney extract):
A typical lot has 10-20 ng/mL of active uPA and
is useful as a uPA activity standard.
Product No.: Human PA 82 Qty.: 0.05 mg
Vitronectin (monomeric form):
This vitronectin was prepared form plasma using
non-denaturing chromatography.
Product No.: Human GP 01 Qty.: 0.5 mg
Vitronectin (multimeric form):
These multimeric vitronectins are made form
plasma using urea as a denaturant.
|
Hu GP02 |
Mo GP04 |
Rat GP05 |
Rab GP06 |
Bov GP03 |
Porc GP07 |
|
0.1 mg |
0.1 mg |
0.1 mg |
0.1 mg |
0.1 mg |