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Cytochrome P450 ReagentsCytochrome b5
Glutathione S Transferase Reagents
Cytochrome P450 enzymes are a superfamily of more than 160 known
members that play a major role in the metabolism of numerous physiological
substrates, as well as, a wide array of xenobiotics including drugs, chemical
carcinogens, insecticides, petroleum products, and other environmental
pollutants. Oxidative metabolism catalyzed by cytochrome P450s can result in
detoxification. In some instances it results in metabolic activation of a
chemical to cytotoxic and/or
carcinogenic forms. Although the liver is the
primary organ for drug metabolism, extrahepatic tissues such as lung, kidney and
intestine, also play an important role for detoxification or biotransformation
of xenobiotics. Each tissue has a unique P450 isozyme distribution and
regulatory mechanism for cytochrome P450 gene (CYP) expression. The expression
of CYPs is increased by several xenobiotics, as well as, endogenous compounds
such as fatty acids and steroids. Expression is altered during development and
by pathophysiological conditions such as starvation and diabetes. P450 protein
levels may be regulated by various mechanisms including; stabilization by
substrate binding, increased translational efficiency of messages, stabilization
of mRNA, enhanced processing of the precursor mRNA, and transcriptional
activation of CYP gene. CYP regulatory mechanisms are diverse and unpredictable.
In many cases, even evolutionary closely related CYPs have been found to be
regulated differently under certain conditions.We have generated antibodies
against all of the major human P450 isoforms. We are also pleased to offer
several purified recombinant human cytochrome P450s, as well as Xenosomes¢â, a
convenient and economical microsomal format containing NADPH-P450
oxidoreductase. Additional reagents, including cDNA probes arealso available for
studies on the regulation of CYP expression. Our product line is organized
according to the CYP family tree for your convenience
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Cytochromes P450 |
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Cytochrome P450 1A Family |
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PR 31 |
Anti-Rabbit CYP450 1A1/1A2 |
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PR 31c |
CYP450 1A1/1A2Preimmune Goat Serum |
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PM 05 |
Anti-Rat CYP450 1A1, Monoclonal |
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PM 10 |
Anti-Rat CYP450 1A1, Monoclonal |
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R 39 |
cDNA Probe, CYP450 1A1 Human |
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PM 17 |
Anti-Human CYP450 1A2, Monoclonal |
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PA 17 |
Anti-Human CYP450 1A2, Rabbit Polyclonal |
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PH 17 |
Anti-Human CYP450 1A2, Goat Polyclonal |
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PH 17c |
CYP450 Goat Preimmune IgG |
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PH 19 |
CYP450 1A2, Recombinat Human |
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R 41 |
cDNA Probe, CYP450 1A2 Human |
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P450 2A Family |
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R 44 |
cDNA Probe, CYP450 2A3 Human |
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PM 19 |
Anti-Human CYP450 2A6, Monoclonal |
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P450 2B Family |
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PM 20 |
Anti-Rat CYP450 2B1/2B2, Monoclonal |
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PM 25 |
Anti-Rat CYP450 2B1, Monoclonal |
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PM 27 |
Anti-Human CYP450 2B6, Monoclonal |
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PR 30 |
Anti-Rabbit CYP450 2B4 |
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PR 30c |
CYP450 2B4 Preimmune Goat Serum |
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CR 62 |
cDNA Probe, Rabbit CYP 2B4, B0 |
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CR 64 |
cDNA Probe, Rabbit CYP 2B4, B1 |
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CR 66 |
cDNA Probe, Rabbit CYP 2B5, B2 |
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CR 68 |
cDNA Probe, Rabbit CYP 2B4/5, BX |
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P450 2C Family |
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PD 24 |
Xenosomes P450 2C9, Recombinant Human (0.5 nmol) |
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PD 25 |
Xenosomes P450 2C19, Recombinant Human 0.5 nmol) |
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PA 22 |
Anti-Human CYP450 2C10, Rabbit Polyclonal |
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R 54 |
cDNA Probe, CYP450 2C9 Human |
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R 56 |
cDNA Probe, CYP450 2C10 Human |
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PM 28 |
Anti-Rat CYP450 2C11, Monoclonal |
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PM 30 |
Anti-Rat CYP450 2C11, Monoclonal |
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PH 40 |
CYP450 2C19, Recombinant Human |
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P450 2D Family |
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PH 46 |
CYP450 2D6, Recombinant Human |
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PD 27 |
Xenosomes P450 2D6, Recombinant Human |
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PA 45 |
Anti-Human CYP450 2D6, Goat Polyclonal |
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CR 98 |
cDNA Probe, Human 2D6 |
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PM 31 |
Anti-Rat CYP450 2D6, Monoclonal |
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P450 2E Family |
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PH 27 |
CYP450 2E1, Recombinant Human (150 ug) |
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PR 32 |
Anti-Rabbit CYP450 2E1 |
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PR 32c |
CYP450 2E1 Preimmune Goat Serum |
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PA 26 |
Anti-Human CYP450 2E1, Rabbit Polyclonal |
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PM 32 |
Anti-Rat CYP450 2E1, Monoclonal |
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PM 33 |
Anti-Rat CYP450 2E1, Monoclonal |
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R 49 |
cDNA Probe, CYP450 2E1 Human |
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PD 32 |
Xenosomes P450 2E1, Recombinant Human (0.5 nmol) |
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P450 3A Family |
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PM 40 |
Anti-Rat CYP450 3A1, Monoclonal |
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PH 34 |
CYP450 3A4, Recombinant Human |
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PD 34 |
Xenosomes P450 3A4, Recombinant Human (0.5 nmol) |
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PA 32 |
Anti-Human CYP450 3A4, Rabbit Polyclonal |
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PM 42 |
Anti-Human CYP450 3A4, Monoclonal |
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R 59 |
cDNA Probe, CYP450 3A4 Human |
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PD 35 |
Xenosomes P450 3A5, Recombinant Human (0.5 nmol) |
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P450 4A Family |
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R 20 |
cDNA Probe, CYP450 4A1Human |
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R 21 |
cDNA Probe, CYP450 4A2 Human |
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R 22 |
cDNA Probe, CYP450 4A3 Human |
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R 23 |
cDNA Probe, CYP450 4A8 Human |
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EZ 91 |
EZ-Prime Rat CYP 4A1 Primers |
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EZ 92 |
EZ-Prime Rat CYP 4A2 Primers |
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EZ 93 |
EZ-Prime Rat CYP 4A3 Primers |
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EZ 94 |
EZ-Prime Rat CYP 4A8 Primers |
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P450 4B Family |
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CR 60 |
cDNA Probe, Rabbit CYP 4B1 |
Recombinant NADPH-Cytochrome P450 Reductase: This recombinant human enzyme is produced from insect cells infected with baculovirus containing the cDNA for human P450 reductase. This product is purified and retains high activity (20-60 µmol cytochrome c reduced/min/mg). Product Number PH 50
Cytochrome b5
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Recombinant cytochrome b5: This membrane bound heme protein enhances the catalytic activity of some cytochrome P450 isoforms, particularly members of the P450 3A family. This product is produced in an E. coli expression system and supplied as a purified, soluble form. Product Number PH 90. |
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Anti-rabbit Cytochrome b5: This goat polyclonal anti-rabbit antibody is specific for cytochrome b5 by immunoassay or Western blot. Sufficient for at least 10 Western blots. A dilution of 1/1000 for Western blots will yield a positive result with 0.2 µg of purified rabbit cytochrome b5. Product Number: PR 91 |
Glutathione S Transferase Reagents
The glutathione S-transferases (GSTs) are a superfamily of inducible enzymes
with overlapping substrate specificities which catalyze the conjugation of
reduced glutathione to various electrophilic substrates. GSTs are abundant in
mammalian livers, and are important in detoxification of many different
xenobiotics in mammals. In addition to conjugating activity, the GSTs bind a
number of hydrophobic compounds such as heme, bilirubin, polycyclic aromatic
hydrocarbons and dexamethasone. The GSTs have been implicated in numerous
aspects of carcinogenesis and anticarcinogenesis. Rat liver GST isozymes are
inducible to varying degrees by a number of xenobiotics, including
phenobarbital, 3-methylcholanthrene, BHA, diphenols, thiocarbamates,
1,2-dithiol-3-thiones, isothiocyanates, cinnamates, and coumarins. Elevated GST
levels have been detected in colonic, hepatic, prostate, lung, breast, ovarian,
gastric and renal cell carcinomas, as well as melanoma. GST has been identified
in the human sera of cancer patients, and levels of this isozyme correlated to
the progression of the disease.
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Glutathione S-Transferases |
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GS 80 |
Anti-Human GST Rabbit Polyclonal |
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Glutathione S- Transferase alpha |
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GS 09 |
Anti-Rat GST Ya Goat Polyclonal |
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GS 10 |
GST Ya, Rat Liver (10 ug) |
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GS 11 |
GST Ya-Yc, Rat Liver (10 ug) |
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GS 60 |
GST A1-1, Recombinant Human (100 ug) |
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GS 62 |
Anti-Human GST A1-1 Rabbit Polyclonal |
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GS 62c |
GST A1-1 Preimmune Control |
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Glutathione S-Transferase mu |
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GS 22 |
GST Yb1, Rat Liver (5 ug) |
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GS 23 |
Anti-Rat GST Yb Goat Polyclonal |
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GS 24 |
GST Yb2, Rat Liver (5 ug) |
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GS 65 |
GST M1-1, Recombinant Human (100 ug) |
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GS 67 |
Anti-Human GST M1-1 |
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GS 67c |
GST M1-1 Preimmune Control |
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Glutathione S-Transferase pi |
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GS 70 |
GST P1-1, Recombinant Human (100 ug) |
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GS 40 |
GST Yp, Rat Liver (100 ug) |
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GS 72 |
Anti-Human GST P1-1 |
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GS 72c |
GST P1-1 Preimmune Control |
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Glutathione S-Transferase Theta |
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GS 50 |
GST T-5-5, Recombinant Rat (100 ug) |
Epoxide Hydrolase
Epoxide hydrolases are involved in the metabolism of a wide range of drugs and other xenobiotics. These enzymes catalyze the addition of water to an epoxide to form the vicinal dihydrodiol, which typically has the trans- configuration. The enzymatic hydration is essentially irreversible and produces metabolites of lower reactivity that can be readily conjugated and excreted. (Seidegard, Ekstrom, Environ. Health Perspectives. 105:791-799, 1997.)
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Anti-Rabbit Epoxide Hydrolase, Goat Polyclonal This antibody, prepared against purified rabbit epoxide hydrolase, is intended for characterization of epoxide hydrolase on Western blots. It also cross-reacts with rat epoxide hydrolase. One vial contains sufficient antibody to perform at least 10 blots following electrophoresis on mini-gels. Product Number PR 35 |
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cDNA Probe for Epoxide Hydrolase (Human) Microsomal epoxide hydrolase is induced by many compounds, including phenobarbital, trans-stilbene oxide and aflatoxin B. Our cDNA probe is 0.91 kb. Product Number R 64 |
Sulfotransferases
The sulfotransferases (STs) are a family of enzymes that catalyze the transfer of sulfate from phosphoadenosine phosphosulfate to some endogenous substances (e.g. hormones and neurotransmitters), as well as, a number of xenobiotics. At least 5 STs are expressed in human liver, all of which are homodimers. Our recombinant human STs are supplied as XenosolTM preparations, which consist of the cytosol fraction from insect cells infected with a baculoviral vector containing a cDNA insert that encodes the specified ST.
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Sulfotransferase 1A2*1: This XenosolTM preparation consists of Sf-9 insect cell cytosol containing recombinant human sulfotransferase 1A2*1 with high catalytic activity. Product Number: ST 18 |
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Sulfotransferase 1A1*2: This XenosolTM preparation consists of Sf-9 insect cell cytosol containing recombinant human sulfotransferase 1A1*2 with high catalytic activity. A typical lot has a specific activity of > 15,000 pmol/min/mg using p-nitrophenol as the substrate. Product Number: ST 10 |
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Sulfotransferase 1A3: This XenosolTM preparation is consists of Sf-9 insect cell cytosol containing recombinant human sulfotransferase 1A3 with high catalytic activity. A typical lot has a specific activity of > 9500 pmol/min/mg using dopamine as substrate. Product Number: ST 12 |
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Anti-Human Sulfotransferase 1A3: This rabbit antisera prepared against recombinant human sulfotransferase 1A3 is suitable for detection of ST1A3 on Western blots and in other immunochemical procedures. One vial contains 400 µL of serum and is sufficient for at least 10 Western blots following electrophoresis on mini-gels. Product Number: ST 13 |
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Sulfotransferase 1E: This XenosolTM preparation consists of Sf-9 insect cell cytosol containing recombinant human sulfotransferase 1E with high catalytic activity. A typical lot has a specific activity of 350 pmol/min/mg using estrone as the substrate. Product Number: ST 14 |
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Anti-Human Sulfotransferase 1E: This rabbit antisera prepared against purified recombinant human sulfotransferase 1E is suitable for detection of ST1E on Western blots and in other immunochemical procedures. One vial contains 400 µL of serum and is sufficient for at least 10 Western blots following electrophoresis on mini-gels. Product Number: ST 15 |
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Sulfotransferase 2A1: This XenosolTM preparation consists of Sf-9 insect cell cytosol containing recombinant human sulfotransferase 2A1 with high catalytic activity. A typical lot has a specific activity of > 700 pmol/min/mg using dihydroepianrosterone (DHEA) as substrate. Product Number: ST 16 |
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Anti-Human Sulfotransferase 2A1: This rabbit antisera prepared against purified recombinant human sulfotransferase 2A1 is suitable for detection of ST2A1 on Western blots and in other immunochemical procedures. One vial contains 400 µL of serum and is sufficient for at least 10 Western blots following electrophoresis on mini-gels. Product Number: ST 17 |
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Control Xenosol for Sulfotransferase Studies: This is a cytosol preparation obtained from sham transfected Sf-9 insect cells. Product Number: ST 20 |
Microsomes
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Microsomes, Fisher (F344) Control Rat Liver: Microsomes from male Fisher (F344) rats. This product is intended to be used as a control for cytochrome P450 enzyme activity assays. Enzyme activity is .747 nmol/min/mg protein (Erythromycin N-demethylase Assay). Total CYP P450 content is 1.0 nmol/mg ppotein. Store at -80°C. Product Number: AM 10 |
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Microsomes, Cornoil Induced Rat Liver: Microsomes from male Sprague Dawley rats treated with cornoil. This product is intended for use as a control for cytochrome P450 enzyme activity assays. Enzyme activity is 1.394 nmol/min/mg protein (Erytromycin N-demethylase Assay).Total P450 content is 0.909 nmol/mg protein, store at -80°C. Product Number: AM 15 |
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Microsomes, Pyridine Induced Rat Liver: Microsomes from male Sprague Dawley rats treated with pyridine to induce high levels of cytochrome P450 2E1. This product has been tested for P450 2E1 activity using P-Nitrophenol Assay. Enzyme activity is 1.15 nmol/min/mg protein. Total CYP P450 content is 1.0 nmol/mg protein. Store at -80°C. Product Number: AM 21 |
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Ram Seminal Vesicles: Ram seminal vesicles are obtained at slaughter and immediately frozen at -70°C. These vesicles are an excellent source for isolation of several eicosanoid metabolizing enzymes. Product Number: V 01 |
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Seminal Vesicle Microsomes: The microsomal fraction of fresh ram seminal vesicles. Useful for enzyme purification or, by addition of inhibitors, as a crude source of specific enzyme activities. Each vial contains 5 mg, which is sufficient for approximately 100 oxygraph `ssays. Product Number: V 02 |